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Job Title:
MacroLab Staff Research Associate (9613C) #25207

Job ID:
25207

Location:
Main Campus-Berkeley

Full/Part Time:
Full-Time

Regular/Temporary:
Regular

Department
QB3 Institute

About Berkeley

The University of California, Berkeley, is one of the world's most iconic teaching and research institutions. Since 1868, Berkeley has fueled a perpetual renaissance, generating unparalleled intellectual, economic and social value in California, the United States and the world. Berkeley's culture of openness, freedom and acceptanceacademic and artistic, political and culturalmake it a very special place for students, faculty and staff.

Berkeley is committed to hiring and developing staff who want to work in a high performing culture that supports the outstanding work of our faculty and students. In deciding whether to apply for a staff position at Berkeley, candidates are strongly encouraged to consider the alignment of the Berkeley Workplace Culture with their potential for success at http://jobs.berkeley.edu/why-berkeley.html.

Application Review Date

The First Review Date for this job is: July 4, 2018.
Position will remain open until filled.

Departmental Overview

The California Institute for Quantitative Biosciences at UC Berkeley (QB3-Berkeley) is the Berkeley home of QB3, a partnership between the state of California, private industry, and the UC campuses at
Berkeley, San Francisco and Santa Cruz. More than 100 faculty members spanning 12 departments at UC Berkeley are affiliated with QB3-Berkeley.

Responsibilities

DNA Cloning/Molecular Biology: Use PCR and standard cloning procedures to construct expression vectors for various proteins, including co-expression vectors to produce protein complexes.

Typical duties include but are not limited to:
  • Amplification of DNA using PCR in up to 96-well format
  • Constructing DNA plasmids using traditional, Gateway and LIC methods
  • Transformation and manipulation of E. coli

    Tasks are assigned by project and vary by project.
    Protein purification: use FPLC systems to purify proteins using defined protocols
  • Expression of proteins in E. coli and yeast
  • Purification of recombinant proteins using defined protocols and FPLC systems
  • Maintaining FPLC systems in good working order
  • Optimization of purification protocols based (in part) on literature and database research
  • Maintaining accurate records and writing clear, concise reports for customers

    Although methods are standardized, selection and optimization of methods for expression and purification must be tailored for each project/protein.
    Laboratory organization:
  • Assisting in ordering and stocking common lab items
  • Keeping lab stocked with commonly used buffers
  • Contributing to general lab safety

    Required Qualifications

  • Bachelor degree in biochemistry, molecular biology or related field or equivalent experience.
  • Previous laboratory experience with protein purification using FPLC systems (e.g. GE AKTA series, Bio-Rad NGC).
  • Knowledge of sterile technique and basic methods of molecular biology and biochemistry.
  • In consultation with the other lab members, the incumbent must make informed decisions requiring consideration of various factors, which presupposes a basic understanding of general biochemistry and molecular biology.
  • Literature research with an emphasis on extracting useful methodological information is critical.
  • The ability to work independently as well as cooperatively as a team member is very important.
  • Attention to detail and good communication skills essential.

    Preferred Qualifications

  • Experience with standard DNA cloning techniques, experience with insect cell expression (e.g. sf9).

    Salary & Benefits

    Hourly Salary Range: $20.74/hour - $23.11/hour

    For information on the comprehensive benefits package offered by the University visit:

    http://ucnet.universityofcalifornia.edu/compensation-and-benefits/index.html

    How to Apply

    Please submit your cover letter and resume as a single attachment when applying.

    Equal Employment Opportunity

    The University of California is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, or protected veteran status. For more information about your rights as an applicant see: http://www.eeoc.gov/employers/upload/poster_screen_reader_optimized.pdf
    For the complete University of California nondiscrimination and affirmative action policy see:
    http://policy.ucop.edu/doc/4000376/NondiscrimAffirmAct

    To Apply, visit:
    https://jobsprod.is.berkeley.edu/psp/jobsprod/EMPLOYEE/HRMS/c/HRS_HRAM.HRS_CE.GBL?Page=HRS_CE_JOB_DTL&Action=A&JobOpeningId=25207&SiteId=1&PostingSeq=1







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