Abalone Bio is a San Francisco Bay Area early-stage biotechnology company developing antibody drugs for G protein-coupled receptors (GPCRs) and other membrane protein targets. We use a proprietary yeast-based discovery engine that directly selects for functional antibodies from natural or synthetic antibody libraries. We are developing our own therapeutics for internal development and co-development with partners, and we are also setting up co-discovery partnerships with big pharma.
In 2018, we isolated the first antibody with full agonist activity for a GPCR. In 2019, we will test this antibody in rodent models of disease. We will also isolate and characterize agonists and antagonists for several additional therapeutic targets. During this time, we also will optimize and scale our approaches to improve discovery speed and efficiency. In 2020 we expect to test antibodies for two additional GPCRs in rodent models of other diseases.
Who are we looking for?
We are looking for an enthusiastic, self-driven science-lover who want to be part of a fun and hardworking startup team. A team player who relates well with others, comfortable with a rapidly changing, demanding startup atmosphere. Good judgment in prioritizing experiments and other responsibilities, and careful record keeping, are essential.
Come join us!
What do we offer?
Compensation include salary commensurate with qualifications, stock options, health, dental and vision coverage and a retirement plan.
Our facilities are located in the East Bay (Richmond, CA). We are fully equipped for molecular biology, microbiology and tissue culture work.
The Scientist will join a small cross-functional R&D team, report to the Chief Scientific Officer, and work closely with other Abalone Bio team members.
At least weekly, the full team discusses experimental results, future plans and priorities. In our meetings, we emphasize respect, inclusiveness, crisp logic and openness. The two founders present and discuss their work on business development, fundraising and pre-clinical and clinical programs.
Advisors and consultants routinely interact with the lab scientists, in the lab, by email or in special meetings.
The growth and development of everyone in the team is an important priority for us. Founders hold regular one-on-one meetings with all team members to ensure their concerns and requests for information are being heard and addressed, and to monitor individually tailored career development paths.
4 or more years of hands-on research lab experience.
Recombinant DNA experience: PCR and cloning. Including design, sequence analysis and bench work.
Computational skills, including experience in data analysis using Excel, R or other analytical tools; scripting in Unix or programming in Python.
Other experience: one or more of the techniques detailed below.
Ability to communicate with others succinctly and effectively about work progress and results, including oral presentations using slides.
Job Description And Specific Responsibilities
Lab work will include some or all of the following types of experiments:
Design, construct and characterize antibody libraries
Design, implement and test modifications to Abalone Bio’s antibody selection technology
Design, construct and screen libraries of GPCR variants for improved performance
Characterize GPCR expression using fluorescent protein microscopy
Perform yeast cell-based selections for functional antibodies against GPCRs
Characterize hits from selections in yeast (plate-based growth curves, other low- and high-throughout assays)
Design, construct and screen libraries of antibody variants for paratope mapping and affinity maturation
Produce recombinant antibodies in E. coli and mammalian cells
● Yeast antibody selections: optimize plating conditions, track and process large sets of culture plates, develop hits through the secondary screening processes.
● Molecular biology: Genetically engineer yeast strains and DNA constructs using yeast recombineering/homologous recombination/CRISPR-based cloning techniques
● Fluorescence techniques: Fluorescence proteins and immunofluorescence measured by fluorescence microscopy and flow cytometry.
● Protein biochemistry: Express recombinant proteins, prepare extracts. Purify using affinity resins (IMAC, protein A) as well ion exchange and size exclusion resins (HPLC). Characterize by SDS-PAGE and Western blot.
● Lab operations: ordering, maintenance of equipment and supply, communal tasks
● Record keeping: Detailed record-keeping of theory, design, bench work and analysis using shared documents in the Google Apps suite and labnote tools (e.g. Benchling). Use of LIMS systems to track samples, data, and related experiments.